Figure 2.

FK506-binding protein 10 (FKBP10) is up-regulated in idiopathic pulmonary fibrosis (IPF). (A) Heat map of FKBP10 gene expression, extracted from microarray data of normal histology control (n = 43) and samples from patients with IPF (n = 99). Every column corresponds to a patient. Increases and decreases are denoted in increasing shades of yellow and purple, respectively, and gray is unchanged (false discovery rate < 5%). (B) Box-and-whisker plot for FKBP10 gene expression data. The difference is highly significant (***P < 1 × 10⁻⁷). (C) Western blot analysis of total lung tissue homogenate showed up-regulation of FKBP10 in patients with IPF relative to donor samples in an independent cohort. (D) Densitometric analysis of the Western blot showed that FKBP10 up-regulation in IPF is highly significant. Data shown are mean ± SEM, and a two-tailed Mann-Whitney test was used for statistical analysis (**P < 0.01). α-SMA = α-smooth muscle actin; ACTB = β-actin as loading control.