Figure 9.

In idiopathic pulmonary fibrosis fibroblasts, FK506-binding protein 10 (FKBP10) knockdown inhibits collagen secretion with similar efficiency as nintedanib, whereas pirfenidone shows no effect. (A–C) Normalized levels of secreted collagen in response to (A) FKBP10 knockdown, (B) nintedanib, and (C) pirfenidone treatment at varying concentrations. In contrast to pirfenidone, nintedanib shows a dose-dependent effect. In comparison, FKBP10 knockdown performs with similar efficiency as submicromolar concentrations of nintedanib. For FKBP10 knockdown, scrambled small interfering RNA (siRNA) was used as control. Data shown are based on four independent experiments and given as mean ± SEM. Statistical analysis was performed using paired two-tailed t test. *P < 0.05, **P < 0.01. ctrl = control; TGF-β = transforming growth factor-β.