Table 1. Strains used in this study.

H. mediterranei strains	Relevant characteristics	Source or reference
DF50	ΔpyrF strain of H. mediterranei ATCC 33500	Liu et al.26
DFA50	trpA deletion mutant of DF50	This study
DF50ΔoriC1-cdc6A	oriC1-cdc6A deletion mutant of DF50	This study
DF50ΔoriC2-cdc6C	oriC2-cdc6C deletion mutant of DF50	This study
DF50ΔoriC3-cdc6G	oriC3-cdc6G deletion mutant of DF50	This study
DF50ΔoriC4-cdc6H	oriC4-cdc6H deletion mutant of DF50	This study
DF50ΔoriP1-cdc6L	oriP1-cdc6L deletion mutant of DF50	This study
DF50ΔoriP2-cdc6M	oriP2-cdc6M deletion mutant of DF50	This study
DF50ΔoriC1	oriC1 deletion mutant of DF50	This study
DF50ΔoriC2	oriC2 deletion mutant of DF50	This study
DF50ΔoriC3	oriC3 deletion mutant of DF50	This study
DF50ΔoriC4	oriC4 deletion mutant of DF50	This study
DF50ΔoriC1ΔoriC2	oriC1 and oriC2 deletion mutant of DF50	This study
DF50ΔoriC1ΔoriC3	oriC1 and oriC3 deletion mutant of DF50	This study
DF50ΔoriC1ΔoriC2ΔoriC3	oriC1, oriC2 and oriC3 deletion mutant of DF50	This study
DF50ΔoriC1ΔoriC2ΔoriC4	oriC1, oriC2 and oriC4 deletion mutant of DF50	This study
DF50ΔoriC1ΔoriC3ΔoriC4	oriC1, oriC3 and oriC4 deletion mutant of DF50	This study
DFA50ΔoriC1ΔoriC2ΔoriC3	oriC1, oriC2, oriC3 and trpA deletion mutant of DF50	This study
DFA50ΔoriC4::trpA	ΔoriC4::trpA mutant of DFA50	This study
DF50R	p.tna-radA mutant of DF50	This study
DF50ΔoriC1ΔoriC2ΔoriC3R	p.tna-radA mutant of DF50ΔoriC1ΔoriC2ΔoriC3	This study
H13	ΔoriC4-cdc6H::trpA-oriP2-orc13 mutant of DFA50ΔoriC1ΔoriC2ΔoriC3	This study