Figure 3. Changes in the expression of circadian and synapse-associated genes after food-deprivation.
(A) Gene expression changes for core circadian genes. Left, mean expression level [log2(TPM)] of each transcript of each experimental group. Right, log2(fold-change) and q-values for differential expression between FD and fed states separately for AGRP and POMC neurons. (B) E-box genes differentially expressed during food-deprivation. (C) DEG with synapse-localized functions (Gene Ontology: 0045202). Top, AGRP neurons: FD vs fed; bottom, POMC neurons FD vs fed. (D, E) Gene expression changes for granin genes (D) and synaptotagmins (E).
Figure 3—figure supplement 1. Arntl/Bmal expression in AGRP neurons.
(A) Representative images for Arntl/Bmal-immunofluorescence in AGRP neurons from fed and FD mice. Scale, 10 µm. (B) Arntl/Bmal immunofluoresence intensity (bars, mean values) in AGRP neurons from fed and FD mice. Fed, n = 159; FD, n = 196; 3 mice per condition.