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. 2015 Oct 7;5:14707. doi: 10.1038/srep14707

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(a) A schematic representation of centromere 1 and the position of a inserted marker gene (otr1R::ura4⁺). (b) Fivefold serial dilution assay to examine the silencing of otr1R::ura4⁺. Wild-type (WT) cells with silenced ura4⁺ grow normally on medium containing 5FOA, while loss of silencing kills cells on 5FOA. (c) RT-PCR analysis of otr1R::ura4⁺ and pericentromeric repeat (dh) RNA levels relative to a control act1⁺. The relative level in WT cells was arbitrarily designated as 1. Each column shown in (c) and below represents the mean ± s.d. from three biological repeats. (d,e) ChIP analysis of enrichment of Pol II (d) and H3K9me2 (e) at otr1R::ura4⁺ and dh relative to fbp1⁺. Relative enrichment in WT cells was arbitrarily designated as 1.