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. 2015 Oct 7;5:14707. doi: 10.1038/srep14707

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Copyright © 2015, Macmillan Publishers Limited

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(a) RT-PCR analysis of RNA levels of representative factors essential for the heterochromatic silencing. The relative level to a control act1⁺ in WT cells was arbitrarily designated as 1. Each column shown in (a) and below represents the mean ± s.d. from three biological repeats. (b) Fivefold serial dilution assay to examine the silencing of otr1R::ura4⁺ in deletion mutants of representative SAGA subunits. (c) RT-PCR analysis of otr1R::ura4⁺ and pericentromeric repeat (dh) RNA levels relative to a control act1⁺. (d) ChIP analysis of enrichment of Spt7-3HA at dh, dg, otr1R::ura4⁺ and mae2⁺ relative to fbp1⁺. Relative enrichment in the cells without tagging (no tag) was arbitrarily designated as 1.