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. 2015 Oct 7;5:14707. doi: 10.1038/srep14707

Figure 4. Sgf73 is required for the integrity and recruitment of RITS.

Figure 4

(a) Co-immunoprecipitation (Co-IP) assay to analyze the physical association between Sgf73 and subunits of RITS. Sgf73-3HA immunoprecipitation (IP) was followed by the Western blot (WB) of Ago1. Chp1-3FLAG IP was followed by the WB of Sgf73-3HA. Input and IP samples were run in the same gel. Cropped blots were shown for clarity. Full-length blots are presented in Supplementary Figs S4 and S5. (b) Co-IP assay to analyze the physical association between subunits of RITS in WT and sgf73Δ cells. Tas3-3FLAG IP was followed by the WB of Ago1 and Chp1. Chp1-3FLAG IP was followed by the WB of Ago1. Input and IP samples were run in the same gel. Cropped blots were shown for clarity. Full-length blots are presented in Supplementary Figs S6 and S7. (c) ChIP analysis of enrichment of Ago1 or Chp1 at dh and otr1R::ura4+ relative to fbp1+. Relative enrichment in the WT cells was arbitrarily designated as 1 Each column represents the mean ± s.d. (n = 3) from three biological repeats.