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. 2015 Oct 7;3:58. doi: 10.3389/fchem.2015.00058

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Copyright © 2015 Magnusson, Appelqvist, Cieślar-Pobuda, Bäck, Kågedal, Jonasson, Los and Nilsson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intracellular targets of p-HTIm. Merged fluorescence images of (A,C) human fibroblasts (AG01518) and (B,D) melanoma cells (SK-MEL-28) stained with p-HTIm (5 μM, 30 min; green) and incubated in fresh medium for 24 h. The cells were stained with fluorescent markers against α-tubulin, autophagosomes (LC3), ER (calnexin), fibronectin, p62, peroxisomes (PMP70), proteasome 20S, PTEN, recycling endosomes (rab11a), Golgi (Golga2), mitochondria (mitotracker), and lysosomes (LAMP-2) (red). Cell nuclei were labeled with DAPI (blue). The color channels are split in (C) and (D) to improve the visualization. Scale bars 20 μm.