Table 1. Summary of Bin-genomes clustered from metagenomic assembly.

Bin-genome ID	Taxonomy	Core gene-basis frequency (%)a	Genome size (Mbp)	No. of gene/ ORFb	% Complete- nessc		No. of contigs	GC content (%)
MS1	Methanosarcinaceae	22.7 ± 2.5	2.21	2155	90	[HQD]	171	44
DB1	Desulfobulbaceae	7.2 ± 0.7	3.51	3188	95	[HQD]	79	54
Bet1	Betaproteobacteria	3.3 ± 0.5	2.59	3020	87	[SD]	487	64
Bac1	Bacteroidetes	3.1 ± 0.3	2.98	2514	96	[HQD]	86	50
DF1	Desulfobacteraceae	2.7 ± 0.5	3.52	3272	90	[HQD]	166	57
Unc1	Unassigned	2.7 ± 1.1	3.60	3081	80	[SD]	81	59
DM1	Desulfuromonadaceae	2.4 ± 0.6	2.86	3361	84	[SD]	908	60
Unc2	Unassigned	2.2 ± 0.9	2.53	2634	82	[SD]	73	36
DB2	Desulfobulbaceae	1.8 ± 0.3	2.49	2217	93	[HQD]	114	50
Bac2	Bacteroidetes	1.7 ± 0.2	4.59	3748	96	[HQD]	218	44
DF2	Desulfobacteraceae	1.5 ± 0.5	3.35	2967	78	[SD]	262	48
Chl1mixd	Chloroflexi etc.	3.9 ± 1.1	3.88	3715	190d	[mix]	302	54
DM2mixd	Geobacteaceae etc.	2.9 ± 0.7	7.73	10512	347d	[mix]	3843	58

^aAveraged frequency within the metagenome was calculated based on the coverage of 16 universal single-copied core genes (Mean ± SD).

^bNumbers of ORFs have potential errors in metagenomic ORF calling because of incomplete assemblies.

^cValues were calculated from frequency of KO assignment to universal single-copied gene family lists (Supplementary Table S3 and S4). Quality of draft genomes (HQD, High-quality draft; SD, Standard draft; mix, mixture of two or more genomes) were assessed by HMP criteria (Supplementary Table S6)²⁶.

^dChl1mix was considered as mixture of two genomes, while DM2mix was considered as mixture of over three genomes. Those bin-genomes were not used for subsequent metabolic network analyses.