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Knockdown of S6K1 isoform-2 blocks SF2/ASF-mediated transformation. (a) Immunoprecipitation (IP)-western blot analysis of S6K1 isoforms in NIH 3T3 cells overexpressing SF2/ASF, after transduction with retroviruses expressing the indicated S6K1 isoform-specific shRNAs. (b) Cells described in a were seeded in soft agar and colonies were counted as in Figure 6b; means ± s.d. are shown. *P = 6×10−8.
