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. 2015 Feb 25;2:15003. doi: 10.1038/hortres.2015.3

Table 3. Kinetic parameters of purified wild-type apple LOX and the Gly567Ala mutant of MdLOX1a.

    Km Vmax kcat kcat/Km
  Substrate (µM) (µmol min−1 mg−1) (s−1) (µM−1 s−1)
LOX1:Md:1a LA 10.35±0.28 1.57±0.07 2.67±0.13 0.26
  LnA 26.65±0.99 3.05±0.14 5.16±0.24 0.19
LOX1:Md:1c LA 40.61±3.10 30.21±0.93 51.14±1.58 1.26
  LnA 70.85±5.48 18.22±2.69 30.86±4.56 0.44
Gly567Ala LA 11.43±1.21 18.74±0.86 31.75±1.45 2.78
  LnA 15.53±0.87 14.82±0.46 25.10±0.77 1.62