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| Mobile phase (%(v/v)) | Elution time (min) |
|||||
|---|---|---|---|---|---|---|
| 0 | 10 | 20 | 30 | 31 | 35 | |
| A | 9 | 10 | 15 | 20 | 9 | 9 |
| B | 91 | 90 | 85 | 80 | 91 | 91 |
Compositions of mobile phase A: 5:95 (v/v) mix of acetonitrile:double distilled water containing 0.05% (v/v) orthophosphoric acid and mobile phase B: a 50:50 (v/v) mix of acetonitrile:double distilled water containing 0.05% (v/v) orthophosphoric acid. The elution method was based on the method of Zhang et al. 38
