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. Author manuscript; available in PMC: 2015 Oct 7.
Published in final edited form as: J Immunol. 2013 Feb 20;190(7):3089–3099. doi: 10.4049/jimmunol.1202408

Figure 2. Naive and Ag-experienced CD8+ T cells differentially localise Csk and Fyn.

Figure 2

Confocal immunofluorescence of resting naïve (A) and Ag-experienced (B) CD8+ T cells stained with anti-TCRβ plus CD8α, DAPI, Csk, pY, Lck, LckpY505, and Fyn, as indicated. A single 2D optical section (along x-y axis) is shown in each panel with colocalisation shown as a merge image of green and red pixels (column 5). Scale bar represents 3 μM (Naïve) and 3.5 μM (Ag-experienced). (C)Tables show values for colocalisation of white (column 2) and green (column 3) pixels. Pearson’s correlation coefficient (Rr) was calculated using Volocity software from 2 independent experiments.