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. Author manuscript; available in PMC: 2015 Oct 7.
Published in final edited form as: J Immunol. 2013 Feb 20;190(7):3089–3099. doi: 10.4049/jimmunol.1202408

Figure 3. Csk and Fyn display bipolar distribution in Ag-experienced CD8+ T cells following TCRβ/CD8α mediated activation.

Figure 3

Confocal immunofluorescence of (A) naïve and (B) Ag-experienced CD8+ T cells stimulated with TCRβ/CD8α for 5 min. Cells were fixed, permeabilised stained for Csk, column 4, and in column 3 for pTyr (top row), Lck (second row), LckpY505 (third row) and Fyn (bottom row). A single 2D optical section (along x-y axis) is shown in each panel, and all red and green colocalised pixels represented as white pixels on a merge image of 3+4. Scale bar represents 3 μM for naïve cells and 3.5 μM for Ag-experienced cells. (C) Tables show values for colocalisation of white (column 2) and green (column 3) pixels. Pearson’s correlation coefficient (Rr) was calculated using Volocity software from at least 2 independent experiments (Rr 3+4 naïve, n=40; Rr 2+3 naive, n=50; Rr 3+4 n=50; Rr 2+3 n=50).