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. 2015 Sep 24;6:8451. doi: 10.1038/ncomms9451

Figure 2. Differentially abundant transcripts in TB-IRIS onset are associated with innate signalling pathways.

Figure 2

(a) Microarray was performed with 26 whole-blood samples (13 TB-IRIS and 13 non-IRIS). One hundred and thirty-eight transcripts (125 genes) were differentially abundant in the whole blood of the TB-IRIS patients at week 2. Transcript intensity values were normalized to the median of all samples. Transcripts are clustered vertically where green represents decreased abundance and red represents increased abundance. Patient arms are clustered horizontally where blue represents non-IRIS and orange represent TB-IRIS. Significance of pathways over-represented by differentially abundant genes was calculated by Fisher's exact test corrected for Benjamini–Hochberg false discovery rate (BH-FDR) (P=0.05). (b) Using an alternative analytical approach by normalizing transcript intensity to its corresponding baseline (week 0) value, 319 transcripts (281 genes) were found to be differentially abundant in TB-IRIS patients. Significance of pathways over-represented by differentially abundant genes was calculated by Fisher's exact test corrected for BH-FDR (P=0.05). One sample was excluded from the analysis, owing to the lack of corresponding baseline.