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. 2015 Aug 18;2(4):ENEURO.0032-15.2015. doi: 10.1523/ENEURO.0032-15.2015

Figure 2.

Figure 2

TNFα and IL1β produced opposite responses on cerebellar Purkinje cells in Atm+/+ and Atm−/−animals. Cyclin A (A–C), γ-H2AX (D, E), cleaved caspase 3 (G–I), and nuclear HDAC4 (J–L) were measured after cytokine injection. ATM deficiency induced increase in these markers in Purkinje cells (PCs; M–P). TNFα significantly reduced cyclin A in Atm+/+PCs (M) and γ-H2AX in Atm−/−PCs (D, E), whereas IL1β significantly induced cyclin A (A, C, M) and HDAC4 nuclear translocation (J, L, P). TNFa had no effect or slightly reduced these markers in Atm−/−PCs. Quantification confirmed these findings (M, N, P). Cleaved caspase 3 signals were similar in all treatment groups (G–I, O). White arrows indicate PCs with respective damage markers. GC, Granule cell layer; ML, molecular layer. Scale bar, 50 µm. n = 3 for each group.