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. 2015 Aug 18;2(4):ENEURO.0032-15.2015. doi: 10.1523/ENEURO.0032-15.2015

Figure 4.

Figure 4

TNFα and IL1β stimulated opposite responses in Atm+/+ and Atm−/− frontal cortexes. Cyclin A (A–D), γ-H2AX (E–H), cleaved caspase 3 (I–L) and nuclear HDAC4 (M–P) were measured after LPS or cytokine injection. ATM deficiency significantly increasedγ-H2AX in PCs (R). LPS treatment failed to significantly increase the expression of all markers (B, F, J, N). TNFα reduced γ-H2AX (G), whereas IL1β increased it (H). By contrast TNFα had little effect on cyclin A (C) or HDAC4 nuclear localization (O), whereas IL1β increased both (D, P) in Atm−/− and Atm+/+frontal cortex (M–P). Cleaved caspase 3 signals were similar in all treatment groups (I–L). White arrows indicate neurons with respective damage markers. Scale bar, 50 µm. n = 3 for each group.