Figure 4.

α-Klotho protein expression and distribution in human endocrine and neuronal tissues: IHC, positive staining (brown) was found in principal cells of the thyroid epithelium (A), islet cells of the pancreas (B), and catecholamine-producing medullary cells of the adrenal gland (C). When brain and spinal cord were stained, primarily neuronal cells were found to express α-Klotho protein, such as in the cerebral cortex (D), Purkinje cells between the molecular and granular layer in the cerebellum (E), motoneurons in the gray matter of the ventral horns of the spinal cord (F), and neuron cell bodies in the ganglia of the myenteric plexus of the intestine (G). Insets are larger magnifications of neuronal cell bodies. n ≥ 5 for each tissue.