The effect of sitting interrupted with 3 minutes of moderate-intensity walking every half hour (SIT+WALK) vs SIT on serum insulin concentrations (A); 3-hour insulin AUC (B); serum C-peptide concentrations (C); 3-hour C-peptide AUC (D); plasma glucose concentrations (E); and 3-hour glucose AUC (F). Unadjusted means ± SE are shown in A, C, and E. AUC results (B, D, and F) are mean ± SE adjusted for randomization order. *, Significantly different, SIT vs SIT+WALK; P < .05.