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. 2015 Oct 7;6:1093. doi: 10.3389/fmicb.2015.01093

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Copyright © 2015 Caruffo, Navarrete, Salgado, Díaz, López, García, Feijóo and Navarrete.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stereoscopic observation of the 5-([4,6-Dichlorotriazin-2-yl] amino) fluorescein hydrochloride (DTAF)-labeled V. anguillarum in zebrafish larvae. (A) 5 dpf wild type (WT) larvae were inoculated by immersion with 10⁷ UFC/ml DTAF stained V. anguillarum: (i) lateral view scheme of the gut; (ii) lateral view of challenged larvae. (B) 5 dpf Tg(Lyz:DsRed)^nz50 larvae inoculated by immersion with 10⁷ UFC/ml DTAF-stained V. anguillarum (challenge) or sterile E3 as a control (control). (C) Quantification of neutrophils migration was performed in the selected area (yellow rectangle).