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. 2015 Oct 7;6:1093. doi: 10.3389/fmicb.2015.01093

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Copyright © 2015 Caruffo, Navarrete, Salgado, Díaz, López, García, Feijóo and Navarrete.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Screening of protective effect of yeast against V. anguillarum challenge in zebrafish larvae. 4 dpf larvae (n = 60) inoculated by immersion with 5 × 10⁶ UFC/ml yeast and challenged the 5 dpf by immersion with 10⁷ UFC/ml V. anguillarum. (A,B) Survival rate (%) at 6 days post-yeast inoculation. (C,D) Life expectancy (average of survival days of larvae). (A,C) Larvae inoculated with different yeasts and challenged with V. anguillarum; C+, control larvae challenged with V. anguillarum. (B,D) larvae inoculated with different yeast; C-, control larvae non-inoculated. The results show the average ±SD of three independent assays. Asterisks (^∗) indicate significant differences with each respective control. Daggers (†) indicate significant differences among protective yeast.