Figure 5.

Modulation of the nicotine-evoked Ca²⁺-transient with PKA disruption. A−C, Bioluminescent Ca²⁺-activity evoked by nicotine with a constitutively activated PKA in mC* transgenic flies (n = 10, A), with downregulated PKA production in R* transgene flies (n = 8, B) and a high level of cAMP with PKA blocked in R* combined with a G_αs*(n = 9, C). D, Bioluminescent image (accumulation time: 120 s) of the nicotinic response in a typical fly of each genotype. E, Total number of photons during the nicotine response in the CCB and in the ML. F, Total duration of the response in the CCB and in the ML. Values are mean ± SEM. Statistics: same as in Figure 2.