Figure 1.

Clotrimazole is neuroprotective specifically in males. A, TTC staining and infarct analysis was performed 24 h after MCAO and presented as the percentage of corrected hemisphere. Clotrimazole administered at the time of reperfusion caused a significant reduction in infarct size in males but not females. Bars represent mean ± SEM. Comparisons made using one-way ANOVA and Tukey’s post hoc analysis; *p = 0.02 B, TRPM2 mRNA expression was measured by qRT-PCR and quantified relative to the housekeeping gene 18s. Data were normalized to male TRPM2 expression levels. No significant differences in TRPM2 mRNA were observed between males and females (Student’s t test; p = 0.12).