Figure 11.

A Tip60 mutant lacking acetyltransferase activity decreases H4K12 acetylation. Hippocampal neurons (21 DIV) were transfected with a catalytically inactive double mutant of Tip60 (Q377E/G380E, abbreviated as Tip60dm). A, B, Representative pair of neuronal nuclei positive (A) and negative (B) for Tip60dm, showing that upon Tip60dm overexpression, the overall staining of H4K12Ac per nucleus is decreased. Scale bars, 2 μm. C, Distributions of nuclear H4K12Ac staining in neurons expressing Tip60dm versus negative control neurons. Each horizontal line represents the average H4K12Ac intensity of a nucleus. All averages were normalized to the mean of the population of Tip60dm-negative neurons. Circles to the left of the distributions indicate the mean and SEM of each population: mean ± SEM (N) is 1.00 ± 0.02 (n = 408) for Tip60dm-negative and 0.62 ± 0.06 (n = 27) for Tip60dm-positive neurons. p = 4*10⁻⁹.