Arc and βSpIVΣ5 interact with Tip60. A, Tip60 interacts with Arc. HEK293T cells were transfected with either Arc in pCDNA3.1 and YFP vector, or Arc-pCDNA3.1 and Tip60-YFP. Tip60-YFP was immunoprecipitated with a mouse anti-GFP antibody, and Arc was detected with a rabbit anti-Arc antibody. B, Tip60 interacts with βSpIVΣ5. HEK293T cells were transfected with either βSpIVΣ5-HA and YFP vector or βSpIVΣ5-HA and Tip60-YFP. Tip60-YFP was immunoprecipitated with a mouse anti-GFP antibody, and βSpIVΣ5-HA was detected with a mouse anti-HA antibody. C, Tip60 interacts with both Arc and βSpIVΣ5. HEK293T cells were transfected with Arc, βSpIVΣ5-HA, and either YFP or Tip60-YFP. Tip60-YFP was immunoprecipitated with a mouse anti-GFP antibody, and Arc or βSpIVΣ5-HA was detected with a rabbit anti-Arc or mouse anti-HA antibody respectively. The asterix (*) denotes an unidentified band.