Supplementary Figure E1.

The heterogeneous pool of CRISPR-gRNA–transfected cells shows high levels of gene editing at the CYBB locus. Different ratios of WT and mutant plasmids were sequenced: (A) 100% WT, (B) 40:60 WT/mutant, and (C) 100% mutant. (D) This provided a standard curve of signal intensities at the T/G mutation site and allowed an estimate of the efficiency of gene conversion within a heterogeneous pool of gene-converted cells. The location of the G > T mutation is highlighted in (A), along with the polypyrimidine tract (underlined) and the splice site (inverted triangle). WT = wild type.