Supplementary Figure E2.

Sequence analysis of single-cell clones derived from the heterogeneous pool of gene-converted cells shows gene editing to be a frequent event. The CYBB loci of 60 single-cell clones were aligned to the parental cell line CGD2 (shown at the top with PAM sites circled and an arrow indicating the G > T mutation). Deletions induced by non-homologous end joining (NHEJ) following the CRISPR-gRNA–directed DSB are shown with dashes.