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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Epilepsia. 2014 Sep 15;55(11):1826–1833. doi: 10.1111/epi.12771

Fig. 1. Spontaneous seizures increase GABARα4 protein expression in DG.

Fig. 1

Western blots of DG protein homogenates of control rats or epileptic rats sacrificed within (A) 3h of a spontaneous seizure (n=6 controls, n=6 SS) or (B) > 24h after last spontaneous seizure (n=3 controls, n=4 No SS) reacted with anti-Alpha4 and anti-Actin antibodies. (C) Densitometry analysis demonstrates that rats with recent seizure activity show ∼23% increase in GABARα4 expression relative to controls (p<0.01) and ∼20% increase relative to epileptic rats without recent spontaneous seizures (p<0.05) using a one-way ANOVA with Tukey's test for multiple comparisons. Normalized data are presented as mean ± SEM and expressed as percent change. (D) The same rats that express increased GABARα4 protein levels in DG following a recent SS (i.e., <3h SS (SS 3H,n=6)) demonstrate a significantly higher seizure frequency (i.e., seizures/day) during the 7-day monitoring period prior to sacrifice relative to the >24h SS group (SS 24H, n=4; *p=0.0139). Significant differences in seizure frequency were detected using a Mann-Whitney test. For all graphs, Error bars, mean±S.E.M.