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SDS–PAGE analysis of CFPS reaction eluates after metal ion affinity chromatography. G-CSF clone Q was expressed both as a standalone protein and as a fusion to the C-terminus of E.coli pGK as a contingency for obtaining adequate amounts of this clone. Lanes (left-to-right): (1) molecular weight standard (green bar denotes 25 kDa); (2) wild-type G-CSF; (3) negative control reaction; (4) D110H; (5) D113H; (6) Clone 757; (7) pGK-Clone Q; (8) Clone Q.
