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. 2015 Oct 6;15:90. doi: 10.1186/s12896-015-0211-3

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© Lee et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Fluorescence image of I⁻-YFP GJIC assay in LN215 cells. The LN215-YFP^QL cells combined with wild-type LN215 (a) or LN215-I^- (b) cells were plated on a 96-well plate. After 24 h of incubation, the culture media were replaced with 100 μL of C-solution. Phase contrast and fluorescence images were collected before and 1 min after adding 100 μL of I-solution. For the composition of the C- and I-solutions, see the I⁻-YFP^QL GJIC assay in the Methods section