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. 2015 Oct 6;15:90. doi: 10.1186/s12896-015-0211-3

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© Lee et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Application of this assay system to HOS cells. HOS-I⁻ and HOS-YFP^QL cells were mixed at a 2:1 ratio and plated on a 96-well plate. After 24 h of incubation, the medium was changed to C-solution containing vehicle or 25 μM CBX, followed by further incubation for 10 min before the 10-s I⁻-YFP assay. The percent YFP quenching at 10 s was plotted against for each well. Z’ factor was calculated as described in the Methods section and shown in the graph