Table 1.
Comparison of the Ca2+-aequorin assay and I−-YFPQL assay
| Ca2+-aequorin assay [18] | I−-YFPQL assay (this report) | |
|---|---|---|
| Signal | Luminescence | Fluorescence |
| Reagent needed for assay | Coelenterazine | None |
| Assay time (sec per well) | 40 | 10 Longer assay time increases Z’ factor. |
| Measuring physiologic event? | Yes (Ca2+) | No (I−) |
| Sources of false positives | ● Adrenergic α1 receptor or TrpV1 channel in donor cells | ● SLC26A4 |
| ● Any target affecting cellular calcium | ● Any target increasing cellular permeability to I− | |
| ● Aequorin | ● YFPQL |