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Tiling Assembly and Cufflinks show similar accuracy predicting known genes inserted in a random test genome. A random test genome containing 80 highly expressed, single-isoform genes was created. Four categories were used, containing 20 genes each: (A) one exon, (B) two exons, (C) three exons, and (D) four exons. The number of reads aligned to each gene was varied to determine the read depth at which each programme failed to accurately predict the genes.
