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. 2015 Oct 7;10(10):e0140120. doi: 10.1371/journal.pone.0140120

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© 2015 Isoda et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — The purified fucosylated (A) and nonfucosylated (B) anti-CD20 IgG1 variants were subjected to nonreducing and reducing 5–20% SDS-PAGE analyses. Lane M: molecular mass marker, lane 1: Y296G, lane 2: Y296E, lane 3: Y296L, lane 4: Y296F, lane 5: Y296D, lane 6: Y296P, lane 7: Y296H, lane 8: Y296W, lane 9: Y296S, lane 10: Y296R, lane 11: Y296N, lane12: Y296V, lane 13: Y296 A, lane 14: Y296I, lane 15: Y296C, lane 16: Y296T, lane 17: Y296K, lane 18: Y296M, and lane 19: Y296Q.