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. Author manuscript; available in PMC: 2015 Oct 7.
Published in final edited form as: Semin Dial. 2013 Jul-Aug;26(4):427–438. doi: 10.1111/sdi.12113

TABLE 3.

Methods, media, incubation and time for optimal bacterial monitoring

Dialysis Water and Fluids* Methods Media Incubation parameters (°C, hours)
CMS Regulation1 Membrane filtration
Spread plate
Dip samplers		 TSA2, TGYE3 35°C, 48 hours
ANS Recommended4 Membrane filtration
Spread plate
Pour plate		 TGEA5, R2A6 17–23°C, 7 days
*

Bicarbonate concentrates – methods, media, and incubation parameters are the same as listed above; however, for the recommended monitoring assay, the media should be supplemented with 4% sodium bicarbonate.

1

The current regulation (5) follows the 2004 AAMI recommendations (6).

2

TSA = Tryptic soy agar.

3

TGYE = Standard methods and plate count agar.

4

The 2011 AAMI recommendations have been updated compared to the 2004 recommendations (8).

5

TGEA = Tryptone Glucose Extract Agar.

6

R2A = Reasoner’s Agar No. 2.