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. Author manuscript; available in PMC: 2015 Oct 7.
Published in final edited form as: Cell Biochem Biophys. 2013 Nov;67(2):363–372. doi: 10.1007/s12013-011-9332-z

Fig. 6.

Fig. 6

Expression of PTEN and related downstream proteins in the hypoxia model of PH. Rats were continuously exposed to 10% oxygen (hypoxia) for 3 weeks to induce PH. Reverse Transcription-PCR and western-blot analysis were performed on lung tissues. a Representative RT-PCR and western blot images of PTEN and related proteins. The RT-PCR image shows a decrease in PTEN expression at the mRNA level. Western-blot images show decreases in PTEN, pPTEN, and p53, and increase in pAkt and cyclin-D1 protein levels. b Quantitative results of the blots. Data represent mean ± SEM (n = 8). *P < 0.05 versus respective normoxic control. Significant decreases in both PTEN and pPTEN was observed when compared with the normoxic controls. The results also show a significant downregulation of p53 and upregulation of cyclin-D1 in the PH tissues