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. Author manuscript; available in PMC: 2015 Oct 7.
Published in final edited form as: J Cell Physiol. 2015 Apr;230(4):842–852. doi: 10.1002/jcp.24812

FIGURE 1. Differential effect of Fbw7α and Fbw7β on PGC-1α protein steady state levels.

FIGURE 1

A. To examine endogenous PGC-1α, Fbw7−/− cells were transfected with empty vector, Fbw7α or Fbw7β. 18h later cells were lysed and evaluated via SDS-PAGE and Western blotting for endogenous PGC-1α and actin (upper panel). Quantification of seven independent experiments (lower panel). B. HeLa, Fbw7−/− or Fbw7+/+ cells were transfected with PGC-1α with or without Fbw7α or Fbw7β. 18h later cells were lysed and evaluated via SDS-PAGE and Western blotting for PGC-1α and actin (as a loading control) (upper panel). Quantification of six independent experiments (lower panel).