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. Author manuscript; available in PMC: 2015 Oct 7.
Published in final edited form as: J Cell Physiol. 2015 Apr;230(4):842–852. doi: 10.1002/jcp.24812

FIGURE 7. Effect of Fbw7α and Fbw7β on ubiquitin conjugates of PGC-1α.

FIGURE 7

Fbw7−/− cells, +/− MG132, were transfected with PGC-1α, Fbw7α, Fbw7β, Fbw7α mutants and HA-ubiquitin, as noted. 18h later, cells were lysed, aliquots were Western blotted with anti-HA (panel A, B) to detect total cellular ubiquitin-protein conjugates or with anti-PGC-1α (panel C, D) to detect total cellular PGC-1α. Additional aliquots were immunoprecipitated with anti-HA and thereafter Western blotted for PGC-1α (panel E) to determine specific ubiquitin-PGC-1α conjugates. Ubiquitin-PGC-1α conjugates are marked by bracket. Low molecular weight conjugates are seen below ~225 kDa.