Table 3.
Detection of anti-WNV antibody in experimentally infected non-native HI birds using the indirect WNV wild bird EIA and the WNV NS1 bEIA
| Species | Sera* N | WNV IgG positive N (%) | WNV NS1 bEIA positive N (%) |
|---|---|---|---|
| HOFI | 10 | 10 (100) | 9 (90) |
| JASP | 13 | 13 (100) | 3 (23†) |
| JAWE | 14 | 14 (100) | 10 (71) |
| COMY | 17 | 17 (100) | 17 (100) |
| SPDO | 18 | 17 (94) | 14 (78) |
| ZEDO | 20 | 20 (100) | 18 (90) |
| Total | 92 | 91 (99) | 71 (77) |
COMY = common mynah; EIA = enzyme-linked immunoassay; HOFI = house finch; HOSP = house sparrow; JASP = Java sparrow; JAWE = Japanese white-eye; RT-PCR = reverse transcriptase polymerase chain reaction; SPDO = spotted dove; WNV = West Nile virus; ZEDO = zebra dove.
*
All sera were obtained ≥ 8 days following WNV challenge and infection. of birds (91 virus culture positive, 1 RT-PCR positive).
†
Proportion of sera positive by WNV IgG EIA significantly greater than by WNV NS1 bEIA (P < 0.001 χ2 test).