Figure 2. Amotl2a is not essential for proneuromast assembly but for proper migration.
(A–F) MIP of Z-stacks of pLLP stained with ZO-1 (blue) and GFP (green) antibodies and phalloidin (red) in control (A, C, E) and amotl2a morphant (B, D, F) cldnb:gfp embryos at 30 hpf. (G, H) MIP of overview images of control (G) and amotl2a morphant (H) embryos after completion of migration. (I, J) Snapshots of time-lapse movies at the indicated timepoints showing a delay in migration in amotl2a morphants (J) as compared to controls (I). (K, L) Corresponding kymographs used to measure the migration speed. (M) Boxplot comparing the migration speeds (Figure 2—source data 1, Figure 2—figure supplements 1, 2).
DOI: http://dx.doi.org/10.7554/eLife.08201.006
Figure 2—source data 1. Migration speed in amotl2a morphants.
elife08201s002.xlsx (40.1KB, xlsx)
DOI: 10.7554/eLife.08201.007
Figure 2—figure supplement 1. Amotl2aMo efficiency.
(A–D) Overview pictures of 30 hpf cldnb:gfp embryos uninjected (A, B) or injected with Amotl2aMo (C, D), imaged either with fluorescent (A–C) or transmitted light (B–D). (E, F) cldnb:gfp embryos injected with RNA encoding the Mo-binding region of Amotl2a fused to GFP (MoBS-amotl2a-gfp) either alone (E) or with Amotl2aMo (F). The bright green fluorescence in F comes from the expression of the cldnb:gfp transgene in the brain and eyes. The red arrowheads in A and C indicate the level of migration at which embryos were fixed for cell counting quantification (see ‘Materials and methods’).
Figure 2—figure supplement 2. cxcr4b and cxcr7b expression are not affected in amotl2a morphants.
Uninjected (A–C and J–L), p53Mo-injected (D–F and M–O), or Amotl2aMo-injected (G–I and P–R) cldnb:gfp embryos stained with a cxcr4b (A–I) or a cxcr7b (J–R) ISH probe and an anti-GFP antibody (C, F, I, L, O, R). Red arrows point to the pLLP.