Figure 3. Amotl2a is required to limit proliferation in the pLLP.
(A–D) MIP of Z-stacks of pLLP in cldnb:gfp embryos injected as indicated. (E, F) Boxplots showing the number of cells in the primordia of indicated groups, normalized to the control group. (G–I′′) MIP of Z-stacks of pLLP in cldnb:gfp embryos stained with EdU and DAPI showing the green (membranes, middle), red (EdU, right), and merge (left) channels. (J, K) Boxplot showing the comparison of the EdU index in the indicated experimental conditions in whole primordia (J) or separately in the leading and trailing region (K) (Figure 3—source data 1–3, Figure 3—figure supplements 1, 2, Figure 3—source data 4).
DOI: http://dx.doi.org/10.7554/eLife.08201.010
Figure 3—source data 1. Cell counts in amotl2a morphants.
elife08201s003.xlsx (44.9KB, xlsx)
DOI: 10.7554/eLife.08201.011
Figure 3—source data 2. Cell counts in rescue experiment of amotl2a morphants.
elife08201s004.xlsx (38.7KB, xlsx)
DOI: 10.7554/eLife.08201.012
Figure 3—source data 3. EdU ratio in the entire pLLP (Excel sheet 1 related to panel J), leading region and trailing region (Excel sheet 2 related to panel K) of amotl2a morphants.
elife08201s005.xlsx (49.4KB, xlsx)
DOI: 10.7554/eLife.08201.013
Figure 3—source data 4. Cell counts in amotl2a-overexpressing embryos.
elife08201s006.xlsx (36.3KB, xlsx)
DOI: 10.7554/eLife.08201.014
Figure 3—figure supplement 1. Successive steps of the automated ‘cell-counting’ algorithm.
(A) Raw image of pLLP in a cldnb:gfp embryo. (B) Enhanced, filtered image using anisotropic diffusion. (C) Segmentation of individual cells. (D) Segmentation mask of the pLLP.
Figure 3—figure supplement 2. Overexpression of amotl2a leads to reduced cell number in the pLLP.
(A, B) MIP of Z-stacks of pLLP in cldnb:gfp embryos injected as indicated. (C) Corresponding boxplot comparing the number of cells in the indicated experimental conditions. (Figure 3—source data 4).