Figure 7. Loss of Lef1 suppresses the increased cell proliferation in amotl2a mutants.

(A–D′) 30 hpf cldnb:gfp embryos with the indicated genetic background stained with a lef1 ISH probe and an anti-GFP antibody (B′, D′). (E) Boxplot showing the expansion of lef1 expression domain in MZamotl2a^−/− embryos. (F–M) MIP of Z-stacks of pLLP in cldnb:gfp embryos with the indicated genotype, either uninjected (F–I) or injected with a Lef1Mo (J–M). (N) Corresponding boxplot comparing pLLP cell counts (Figure 7—source data 1, 2; Figure 7—figure supplement 1, Figure 7—source data 3).

DOI: http://dx.doi.org/10.7554/eLife.08201.036

Figure 7—figure supplement 1. Loss of Amotl2a leads to an expansion of lef1 expression.

(A–D) MIP of confocal stacks of the pLLP in Tg(7xTCF-Xla.Sia:NLS-mCherry) embryos injected (B, D) or not injected (A, C) with the amotl2aMo and showing the green (membranes, left) and red (nuclear WNT reporter) channels. (E) Boxplot comparing the relative mean intensity of fluorescence in the red channel in the corresponding pLLP. (F–I′) Overview pictures (F, H) and close-up on the pLLP of 30 hpf cldb:gfp embryos injected (H, I, I′) or not injected (F, G, G′) with the Amotl2aMo and stained with a lef1 ISH probe and an anti-GFP antibody (G′, I′). Red arrows point to the pLLP (F, H). (J) Boxplot showing the expansion of lef1 expression domain in amotl2a morphants. (K–N) Close-up on the pLLP of 30 hpf control or MZyap1^−/− cldb:gfp embryos stained with a lef1 ISH probe and an anti-GFP antibody (L, N). The relative lef1 expression domain is not different in MZyap1^−/− embryos (Figure 7—source data 3).

DOI: http://dx.doi.org/10.7554/eLife.08201.040