Figure 3.

CENP-A densely occupies central domains, except at tRNA genes. (A) N-ChIP profiles of CENP-A-FLAG are shown for all three centromeric and pericentric regions. Annotations for tRNA genes, noncoding RNAs (ncRNAs), and repeats are indicated on the x-axis, and the y-axis shows fold-enrichment relative to input. (B) CENP-A-FLAG N-ChIP fragments and input fragments were divided into <110-bp and >110-bp size classes. Note that large and small CENP-A ChIP fragments, but not input fragments, show similar profiles. tRNA genes are indicated below the tracks, with those above the red line transcribed left to right and those below transcribed from right to left. Insets show expansions of regions in cen1 and cen2 indicated by brackets.