Figure 8.

Tests of activation at shorter and longer distances. (A) Northern analysis was performed to test the polygenic mutants for activation of a reporter with a shorter distance of 280 bp and to compare the relative levels of activation to the long-distance reporter with a distance of 799 bp. (B) Spot tests were done to measure the level of 3AT resistance in strains that carry reporters with four different distances between the GAL1 UAS and the HIS3 TATA: 280 bp, 799 bp, 1397 bp, and 2027 bp. For reasons we do not understand, we reproducibly observed poor growth of the sin4Δ mutant with the 2027-bp reporter on complete medium. All plates contain galactose as the carbon source. Plates were incubated at 30° for 3 days.