Figure 5.

DAF-16A and DAF-16F target genes identified by whole transcriptome profiling. (A) Depiction of the A-index (I_A) for three hypothetical target genes with I_A = 0, 0.5, and 1.0. Idealized expression profiles in daf-2(e1370), daf-16a/f;daf-2, and daf-16a;daf-2 are shown for all three genes. (B) Scatterplot comparing I_A and I_F for DAF-16A/F target genes. Dashed lines indicate I_A and I_F = 0 or 1. Only genes with indices from −0.2 to 1.2 are shown; a scatterplot with a wider range of indices is shown in Figure S15. I_A and I_F for all genes are listed in Table S13. (C and D) Plots of I_A and I_F for all DAF-16A/F target genes from lowest to highest I_A (C) or I_F (D). Solid lines correspond to indices of 0 and 1. Three genes with indices >2.2 or < −1.2 were omitted for presentation purposes. Gene rankings are listed in Table S13. (E) Tree diagram summarizing the categorization system for DAF-16A and DAF-16F target genes. See text and Materials and Methods for details. (F) Scatterplot from B, with individual genes color coded according to categories depicted in E. Dashed lines indicate I_A or I_F values of 0.2 or 0.8, corresponding to the cutoffs used to define redundantly regulated, A-specific, and F-specific targets.