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. 2015 Aug 10;201(2):631–649. doi: 10.1534/genetics.115.180018

Figure 5.

Figure 5

Blocking endocytic maturation produces enlarged endosomes. (A–E) The following strains were fixed and stained to reveal the actin cytoskeleton: con shRNA (A), rab5 Q88L (B and C), and rab7 shRNA (D and E). The shRNAs and the rab5 Q88L construct were expressed using the maternal α-tubulin-Gal4 driver. The autofluorescent yolk particles are displayed using a color-coded range indicator. DIC images of these egg chambers are in A′, B′, C′, and D′. A stage 14 egg chamber expressing rab7 shRNA is shown in E. The efficacy of Rab5 and Rab7 depletion is shown in Figure S1, A–D. (F and G) Egg chambers from a strain expressing TagRFPt-2xFYVE (F) or from a strain coexpressing dmn shRNA-A and TagRFPt-2xFYVE (G) were fixed and stained to reveal the actin cytoskeleton (green). The inset in D′ shows enlarged endosomes that are present in the Dmn-depleted strain (G′). (H) Egg chambers from a strain coexpressing rab5 shRNA and TagRFPt-2xFYVE were fixed and stained to reveal the actin cytoskeleton (green). (I) Egg chambers from a strain coexpressing rab5 Q88L and TagRFPt-2xFYVE were fixed and stained to reveal the actin cytoskeleton (green). The enlarged RFP-positive vesicles observed upon overexpression of constitutively active Rab5 are indicated (I′). Bar, 50 μm.