Figure 5.

The difference between the hk21 and exon 1A deletion phenotypes. In most embryos produced by cnn^hk21 mutant females, meiotic-like spindles persist at the cortex and polar bodies fail to form (top, a–d and B). Spindles are scattered in space and are relatively rare and nuclear fusions are common (A). Embryos typically fail early, probably due to aneuploidy and failure to form centrosomes. Unlike the cnn^hk21 phenotype, embryos produced by Dp(2; 3)BAC•cnn ^ΔEx1A undergo many rounds of division and nuclei cover the cortex (C–E). Tubulin foci are present at prophase (C) and metaphase (D) but do not appear to organize the meiotic-like spindle poles. Initially nuclei appear to be haploid but as numbers increase fusions occur during metaphase and anaphase (F–H). Polar bodies and persistent meiotic spindles are not observed in these animals similar to the loss of Polo kinase phenotype. Cnn-LF protein is not detected in either embryonic genotype. Cnn-LF is shown in green, tubulin in red, and DNA in blue. For Western analyses of these genotypes see Figure 2.