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(A) Summary of putative minus NESs (in the Dbase data set that match class 1-R patterns exclusively) tested for CRM1 binding. Nap1p (*) was previously shown to direct nuclear export in cells even though no CRM1 binding was observed (Xu et al., 2015). (B) Putative minus NESs that bind CRM1 in pull-down assays with CRM1 and RanGTP. (C) Putative minus NESs that show no observable CRM1 binding.
