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. 2015 Oct 7;10(10):e0140192. doi: 10.1371/journal.pone.0140192

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© 2015 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) Representative PCR genotyping of E3.5 blastocysts from Med28 ^+/- intercrosses show WT (+) allele and null (-) allele. Primers described in Fig 1C are used for genotyping. (B) Phase contrast microscopy images of embryos from Med28 ^+/- intercrosses. At embryonic day E3.5, Med28 ^+/+ blastocysts (panel A; a, enlarged inset) are indistinguishable from Med28 ^-/- blastocysts (panel B; b, enlarged inset). After 3 days in culture (DIV3), unlike Med28 ^+/+ blastocysts that show outgrowth (panel C, arrow), ICMs from Med28 ^-/- blastocysts fail to show outgrowth (panel D). A total of 14 WT and 12 mutant blastocysts were examined. Scale bar, 100 μm.