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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Cancer Immunol Res. 2015 May 12;3(10):1165–1174. doi: 10.1158/2326-6066.CIR-15-0061

Figure 2. Group A EPN patients have higher levels of IL6 production and a higher ratio of pSTAT3 than Group B patients.

Figure 2

(A) Concentration of IL6 secretion from flow-sorted disaggregated tumor samples is higher in group A (FC=3.5; p=0.031, 1-tailed t-test). Cells were cultured for 96hours in O15 and normalized to 106 cells (B) Representative Western blot showing expression of pSTAT3 and Total STAT3 in patient samples. (C) Western blot validation of pSTAT3 activation in surgical samples from 12 Group A and 12 Group B EPN patients. The ratio of pSTAT3 to Total STAT3 was determined by densitometric measurement of western blot bands. This demonstrated a higher ratio of STAT3 phosphorylation in samples from group A EPN patients (FC=1.84; p=0.012).